6IKN

Crystal structure of the GAS7 F-BAR domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-23
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.97890
Spacegroup name	P 1
Unit cell lengths	53.264, 87.281, 88.793
Unit cell angles	80.98, 74.72, 79.73

Refinement procedure

Resolution	32.029 - 3.000
R-factor	0.197
Rwork	0.194
R-free	0.25470
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.934
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	autoSHARP
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.050
High resolution limit [Å]	3.000	3.000
Number of reflections	30444	1515
<I/σ(I)>	7
Completeness [%]	99.1
Redundancy	6.4
CC(1/2)	0.970	0.490

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	PEG 4000, PEG 8000, ammonium sulfate, 80 mM HEPES-NaOH (pH 7.5), MES (pH 6.5)