6IKK
Crystal structure of YfiB(L43P) in complex with YfiR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.798, 58.413, 70.324 |
| Unit cell angles | 72.69, 83.01, 89.33 |
Refinement procedure
| Resolution | 66.620 - 2.190 |
| R-factor | 0.18015 |
| Rwork | 0.179 |
| R-free | 0.20335 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zhu 4zhw |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.543 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.620 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.089 | 0.462 |
| Number of reflections | 37342 | 1797 |
| <I/σ(I)> | 13 | 3.6 |
| Completeness [%] | 97.7 | 95 |
| Redundancy | 3.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10%(w/v) PEG 6000, 0.1 M Bicine pH 9.0 |
