6IKI
Crystal structure of YfiB(W55L)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-30 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 41 |
| Unit cell lengths | 44.563, 44.563, 154.934 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.560 - 2.204 |
| R-factor | 0.2067 |
| Rwork | 0.202 |
| R-free | 0.24940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zhw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.983 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.560 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.070 | 0.452 |
| Number of reflections | 15242 | 764 |
| <I/σ(I)> | 36.8 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% (w/v) PEG-400, 0.1M cacodylate pH 6.5, 0.2M Li2SO4, 7.5%(w/v) glycerol |
