6IK6

Crystal structure of Tomato beta-galactosidase (TBG) 4 with beta-1,4-galactobiose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-12-09
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.000000
Spacegroup name	P 21 21 21
Unit cell lengths	94.032, 110.730, 162.141
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.570 - 2.791
R-factor	0.2002
Rwork	0.196
R-free	0.27980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3w5g
RMSD bond length	0.010
RMSD bond angle	1.172
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.790	2.790
Rmerge	0.127	0.615
Rmeas	0.193	0.928
Rpim	0.072	0.364
Number of reflections	42564	3817
<I/σ(I)>	11
Completeness [%]	99.2	93.83
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	277	16% (w/v) PEG10000, 0.1M HEPES