6IJF
Crystal structure of the type VI effector-immunity complex (Tae4-Tai4) from Agrobacterium tumefaciens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-16 |
| Detector | RAYONIX MX225-HS |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 61 |
| Unit cell lengths | 72.025, 72.025, 194.348 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 62.380 - 1.900 |
| R-factor | 0.19142 |
| Rwork | 0.190 |
| R-free | 0.21252 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bi8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.572 |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.5.26) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 97.180 | 97.180 | 1.940 |
| High resolution limit [Å] | 1.900 | 9.110 | 1.900 |
| Rmerge | 0.067 | 0.041 | 1.431 |
| Rmeas | 0.070 | 0.043 | 1.538 |
| Rpim | 0.022 | 0.014 | 0.544 |
| Total number of observations | 452487 | ||
| Number of reflections | 44633 | 418 | 2768 |
| <I/σ(I)> | 15 | ||
| Completeness [%] | 99.6 | 97.1 | 96.3 |
| Redundancy | 10.1 | 9.4 | 7.3 |
| CC(1/2) | 0.999 | 0.999 | 0.528 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 35 % PEG 400, 0.05 M Tris pH 8.5, 0.05 M sodium sulfate, 0.05 M lithium sulfate |
