6IJC
Structure of MMPA-CoA dehydrogenase from Roseovarius nubinhibens ISM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-01-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.012, 103.429, 125.963 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.970 - 2.300 |
R-factor | 0.1774 |
Rwork | 0.175 |
R-free | 0.22590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4y9j |
RMSD bond length | 0.008 |
RMSD bond angle | 0.893 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.970 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.101 | 0.478 |
Number of reflections | 47323 | |
<I/σ(I)> | 19.07 | |
Completeness [%] | 99.4 | |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Bis-Tris propane, PEG 3350, Sodium nitrate |