6IJA

Crystal Structure of Arabidopsis thaliana UGT89C1 complexed with UDP-L-rhamnose

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	100
Detector technology	CCD
Collection date	2018-03-24
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97891
Spacegroup name	P 1 21 1
Unit cell lengths	90.671, 84.983, 125.472
Unit cell angles	90.00, 89.92, 90.00

Refinement procedure

Resolution	45.335 - 3.214
R-factor	0.2196
Rwork	0.216
R-free	0.29070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ij7
RMSD bond length	0.011
RMSD bond angle	1.370
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.310
High resolution limit [Å]	3.200	6.890	3.200
Rmerge	0.092	0.033	0.775
Rmeas	0.099	0.036	0.837
Rpim	0.038	0.014	0.315
Total number of observations	210757
Number of reflections	31198	3213	3100
<I/σ(I)>	6
Completeness [%]	99.8	99.6	100
Redundancy	6.8	6.5	7
CC(1/2)		0.997	0.777

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1M HEPES pH 7.5, 0.8M potassium phosphate dibasic and 0.8M sodium phosphate monobasic