6IJA
Crystal Structure of Arabidopsis thaliana UGT89C1 complexed with UDP-L-rhamnose
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL18U |
| Synchrotron site | NFPSS |
| Beamline | BL18U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-03-24 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97891 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.671, 84.983, 125.472 |
| Unit cell angles | 90.00, 89.92, 90.00 |
Refinement procedure
| Resolution | 45.335 - 3.214 |
| R-factor | 0.2196 |
| Rwork | 0.216 |
| R-free | 0.29070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ij7 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.370 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 6.890 | 3.200 |
| Rmerge | 0.092 | 0.033 | 0.775 |
| Rmeas | 0.099 | 0.036 | 0.837 |
| Rpim | 0.038 | 0.014 | 0.315 |
| Total number of observations | 210757 | ||
| Number of reflections | 31198 | 3213 | 3100 |
| <I/σ(I)> | 6 | ||
| Completeness [%] | 99.8 | 99.6 | 100 |
| Redundancy | 6.8 | 6.5 | 7 |
| CC(1/2) | 0.997 | 0.777 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M HEPES pH 7.5, 0.8M potassium phosphate dibasic and 0.8M sodium phosphate monobasic |
