6IIR
Complex structure of the HRP3 PWWP domain with a 10-bp GC-rich DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2016-10-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 31 |
Unit cell lengths | 33.554, 33.554, 197.913 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.059 - 2.200 |
R-factor | 0.2049 |
Rwork | 0.202 |
R-free | 0.25770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6iip |
RMSD bond length | 0.009 |
RMSD bond angle | 1.166 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.111 | 0.975 |
Number of reflections | 12832 | 700 |
<I/σ(I)> | 35.9 | 2.1 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.5 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1 M Tris, pH 8.0, 0.1 M sodium malonate, pH 8.0, and 26% polyethylene glycol 1000 |