6IIP

Apo-form structure of the HRP3 PWWP domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	77
Detector technology	PIXEL
Collection date	2016-12-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91531
Spacegroup name	P 21 21 21
Unit cell lengths	38.478, 39.931, 97.385
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.876 - 0.951
R-factor	0.1969
Rwork	0.197
R-free	0.20500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3eae
RMSD bond length	0.006
RMSD bond angle	1.162
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	0.970
High resolution limit [Å]	0.950	0.950
Rmerge	0.072	0.455
Number of reflections	93005	4036
<I/σ(I)>	52.6	5.3
Completeness [%]	97.4	86
Redundancy	11.9	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.2 M ammonium sulphate, 0.1 M MES monohydrate, pH 6.5, and 30% w/v polyethylene glycol monomethyl ether 5000