6II9
Crystal structure of H7 hemagglutinin from A/Anhui/1/2013 in complex with a human neutralizing antibody L3A-44
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97930 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 178.457, 184.906, 188.990 |
| Unit cell angles | 90.00, 106.47, 90.00 |
Refinement procedure
| Resolution | 43.841 - 3.500 |
| R-factor | 0.2024 |
| Rwork | 0.201 |
| R-free | 0.23380 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.994 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.630 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rpim | 0.111 | |
| Number of reflections | 73039 | |
| <I/σ(I)> | 8.4 | 1.6 |
| Completeness [%] | 98.0 | |
| Redundancy | 4.1 | |
| CC(1/2) | 0.977 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.5 | 291.15 | 0.2M Magnesium chloride hexahydrate, 0.1M HEPES pH 7.5, 22% w/v poly (acrylic acid sodium salt) 5100 |
