6II9
Crystal structure of H7 hemagglutinin from A/Anhui/1/2013 in complex with a human neutralizing antibody L3A-44
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97930 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 178.457, 184.906, 188.990 |
Unit cell angles | 90.00, 106.47, 90.00 |
Refinement procedure
Resolution | 43.841 - 3.500 |
R-factor | 0.2024 |
Rwork | 0.201 |
R-free | 0.23380 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.994 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.630 |
High resolution limit [Å] | 3.500 | 3.500 |
Rpim | 0.111 | |
Number of reflections | 73039 | |
<I/σ(I)> | 8.4 | 1.6 |
Completeness [%] | 98.0 | |
Redundancy | 4.1 | |
CC(1/2) | 0.977 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7.5 | 291.15 | 0.2M Magnesium chloride hexahydrate, 0.1M HEPES pH 7.5, 22% w/v poly (acrylic acid sodium salt) 5100 |