6IHS
Crystal structure of bacterial serine phosphatase with His-tag mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9735 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 77.793, 86.355, 38.896 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.400 |
| R-factor | 0.13204 |
| Rwork | 0.130 |
| R-free | 0.16792 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5f1m |
| RMSD bond length | 0.031 |
| RMSD bond angle | 2.587 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.062 | 0.557 |
| Number of reflections | 52150 | 4913 |
| <I/σ(I)> | 44.8 | |
| Completeness [%] | 99.3 | |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M MgCl2, 0.1 M Tris-HCl (pH=8.0), 30% PEG 4000. |
