6IHL
Crystal structure of bacterial serine phosphatase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9735 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.669, 38.160, 64.891 |
| Unit cell angles | 90.00, 101.47, 90.00 |
Refinement procedure
| Resolution | 45.738 - 1.573 |
| R-factor | 0.1715 |
| Rwork | 0.170 |
| R-free | 0.20460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5f1m |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.752 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.087 | 0.496 |
| Number of reflections | 31074 | 2993 |
| <I/σ(I)> | 32.7 | |
| Completeness [%] | 99.4 | |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.05 M MgCl2, 0.1 M HEPES (pH=7.5), 30% PEG 3350 |
