6IGV

Kif5b stalk I coiled-coil

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97778
Spacegroup name	I 41 2 2
Unit cell lengths	84.620, 84.620, 313.912
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.571 - 2.999
R-factor	0.2886
Rwork	0.286
R-free	0.30880
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CRANK2
Refinement software	PHENIX (1.14_3228)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.050
High resolution limit [Å]	2.999	3.000
Rmerge	0.183
Rpim	0.041	0.214
Number of reflections	15845	11994
<I/σ(I)>	15.9	4.68
Completeness [%]	83.9	37.75
Redundancy	18.5	14.3
CC(1/2)	0.988	0.968

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	298	0.2M Ammonium Sulfate, 40%MPD, 0.1M sodium cacodylate pH6.6