6IGU
Crystal structure of the hydrolytic antibody Fab 9C10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 154.436, 60.142, 56.080 |
| Unit cell angles | 90.00, 108.43, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.110 |
| R-factor | 0.17804 |
| Rwork | 0.175 |
| R-free | 0.23691 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dtm |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.878 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rpim | 0.047 | 0.344 |
| Number of reflections | 27974 | 1395 |
| <I/σ(I)> | 16.88 | 2.32 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 30% PEG MME 550, 0.05M calcium chloride, 0.1M Bis-Tris pH 6.5 |
