6IGH

Crystal structure of FT condition3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-08
Detector	RAYONIX MX300HE
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	48.889, 60.956, 63.799
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.740 - 1.010
R-factor	0.1179
Rwork	0.117
R-free	0.13850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wkp
RMSD bond length	0.015
RMSD bond angle	1.473
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.030
High resolution limit [Å]	1.010	1.010
Number of reflections	97694	6267
<I/σ(I)>	9.8
Completeness [%]	97.4
Redundancy	7.4
CC(1/2)	0.994	0.833

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG4000 and ammonium citrate/citric acid, pH 5.5