6IGG
Crystal structure of FT condition 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-06-16 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.618, 48.715, 73.571 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.740 - 1.000 |
R-factor | 0.1172 |
Rwork | 0.116 |
R-free | 0.13560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wkp |
RMSD bond length | 0.013 |
RMSD bond angle | 1.332 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.020 |
High resolution limit [Å] | 1.000 | 1.000 |
Number of reflections | 75289 | 3370 |
<I/σ(I)> | 11.9 | 1.2 |
Completeness [%] | 97.1 | |
Redundancy | 5.7 | |
CC(1/2) | 0.998 | 0.655 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG1500, MPD, and 0.1 M Tris-HCl, pH 8.5 |