6IGF

Crystal structure of Human Papillomavirus type 52 pentamer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	CCD
Collection date	2016-07-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	306.768, 105.070, 196.903
Unit cell angles	90.00, 125.80, 90.00

Refinement procedure

Resolution	42.950 - 2.751
R-factor	0.1893
Rwork	0.187
R-free	0.23520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5y9e
RMSD bond length	0.006
RMSD bond angle	0.924
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.750	2.750
Number of reflections	131803	6516
<I/σ(I)>	12.1
Completeness [%]	99.9
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	8% tasimate, 17.5% (w/v) PEG 3350