Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2016-07-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 136.543, 209.761, 212.630 |
| Unit cell angles | 60.50, 85.07, 90.12 |
Refinement procedure
| Resolution | 41.551 - 3.500 |
| R-factor | 0.3162 |
| Rwork | 0.315 |
| R-free | 0.34020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5y9e |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.616 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.560 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 248099 | 11253 |
| <I/σ(I)> | 4.8 | |
| Completeness [%] | 97.0 | |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2M calcium acetate, 18% (w/v) PEG 3350 |
