6IG5
Crystal structure of argininosuccinate lyase from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-09 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97776 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 115.349, 120.352, 155.422 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.586 - 2.078 |
| R-factor | 0.168 |
| Rwork | 0.166 |
| R-free | 0.19960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hy1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.077 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.078 | 2.100 |
| Rmerge | 0.136 | 0.742 |
| Number of reflections | 128496 | 6334 |
| <I/σ(I)> | 13.6 | |
| Completeness [%] | 99.6 | |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 8% (v/v) tacsimate, 28% (w/v) polyethylene glycol 3350 |
