6IFV
C-terminal truncated KsgA from Bacillus subtilis 168
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 77.867, 77.867, 143.638 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.877 - 3.110 |
| R-factor | 0.19691 |
| Rwork | 0.186 |
| R-free | 0.27400 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.552 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.800 | 3.110 |
| High resolution limit [Å] | 3.100 | 3.110 |
| Number of reflections | 8452 | |
| <I/σ(I)> | 13.1 | 3.7 |
| Completeness [%] | 99.8 | 99.2 |
| Redundancy | 12.2 | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M HEPES sodium pH 7.5, 1.4M Sodium citrate tribasic dihydrate |
