6IFM
Crystal structure of DNA bound VapBC from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2018-05-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 31 |
| Unit cell lengths | 93.677, 93.677, 122.440 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.867 - 2.804 |
| R-factor | 0.2105 |
| Rwork | 0.206 |
| R-free | 0.23310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.089 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.886 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.050 | 0.530 |
| Number of reflections | 29329 | 9280 |
| <I/σ(I)> | 13.3 | |
| Completeness [%] | 99.0 | 97.9 |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M ammonium citrate tribasic pH7, 20% w/v PEG 3350 |
