6IFH

Unphosphorylated Spo0F from Paenisporosarcina sp. TG-14

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	200
Detector technology	CCD
Collection date	2016-05-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	29.518, 49.361, 67.067
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.750 - 1.800
R-factor	0.18939
Rwork	0.188
R-free	0.22594
RMSD bond length	0.011
RMSD bond angle	1.576
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.8.0232)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.800	1.800
Number of reflections	57616
<I/σ(I)>	27.9
Completeness [%]	99.5
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	40% (v/v) PEG 300, 100 mM sodium carcodylate/HCl (pH 6.5), and 200 mM calcium acetate