6IFE
A Glycoside Hydrolase Family 43 beta-Xylosidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.637, 106.135, 105.884 |
| Unit cell angles | 90.00, 122.51, 90.00 |
Refinement procedure
| Resolution | 40.240 - 1.804 |
| R-factor | 0.1618 |
| Rwork | 0.161 |
| R-free | 0.19910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yif |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.019 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.290 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 | 0.572 |
| Number of reflections | 74733 | 7222 |
| <I/σ(I)> | 14.3 | 3.4 |
| Completeness [%] | 70.5 | 92.1 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M Tris pH=8.5, 30% PEG4000, 0.2 M LiCl |
