6IEY

Crystal structure of Chloramphenicol-Metabolizaing Enzyme EstDL136-Chloramphenicol complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-26
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	P 21 21 2
Unit cell lengths	118.463, 152.411, 44.137
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.040 - 2.097
R-factor	0.211
Rwork	0.209
R-free	0.26140
RMSD bond length	0.008
RMSD bond angle	1.161
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.093	2.093
Number of reflections	46963
<I/σ(I)>	263
Completeness [%]	96.4
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	500mM Ammonium fluoride (pH6.5), 30% PEG 3350, 5% glyceol and 120mM TCEP.