6IEW
The crystal structure of the dNxf2 UBA domain in complex with Panoramix
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 29.074, 40.925, 83.091 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.074 - 1.500 |
| R-factor | 0.1647 |
| Rwork | 0.164 |
| R-free | 0.17320 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.640 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.140 | 0.280 |
| Number of reflections | 16378 | 1469 |
| <I/σ(I)> | 21.7 | 3.8 |
| Completeness [%] | 98.8 | 90.3 |
| Redundancy | 9.9 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 30%(w/v) PEG3350, 0.1M Tris (pH 9.0), 0.2M sodium acetate trihydrate |
