6IEP
GAPDH of Streptococcus agalactiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-18 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.580, 124.420, 125.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k73 |
| RMSD bond length | 0.007 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.170 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.157 | 0.430 |
| Rmeas | 0.170 | 0.475 |
| Rpim | 0.049 | 0.152 |
| Number of reflections | 43229 | |
| <I/σ(I)> | 13 | 5.7 |
| Completeness [%] | 99.9 | |
| Redundancy | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 287 | 40% PEG 4000 monomethyl ether and 100 mM Tris-HCl pH 9.0 |
