6IEJ

The C2 domain of cytosolic phospholipase A2 alpha bound to phosphatidylcholine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	C 2 2 2
Unit cell lengths	108.294, 187.388, 68.754
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.881 - 2.206
R-factor	0.2256
Rwork	0.224
R-free	0.24890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rlw
RMSD bond length	0.008
RMSD bond angle	1.117
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Number of reflections	35668
<I/σ(I)>	30.9
Completeness [%]	99.5
Redundancy	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	100 mM HEPES-NaOH buffer, 1.4 M magnesium chloride, 0.6 M sodium chloride