6IEI
Loop deletion and proline insertion mutant (deleting six residues and inserted five proline residues)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-11 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 79.381, 79.381, 105.985 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.22291 |
| Rwork | 0.220 |
| R-free | 0.27558 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g8c |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.526 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.117 | 0.516 |
| Number of reflections | 28975 | 1401 |
| <I/σ(I)> | 28.4 | 1.89 |
| Completeness [%] | 99.9 | 97.6 |
| Redundancy | 9.8 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 292 | 2.6 M Ammonium sulfate, 0.1 M Sodium acetate |
