6IEC

Structure of RVFV Gn and human monoclonal antibody R17

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97853
Spacegroup name	P 62
Unit cell lengths	198.009, 198.009, 164.467
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.560 - 3.200
R-factor	0.2428
Rwork	0.241
R-free	0.28080
RMSD bond length	0.003
RMSD bond angle	0.707
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.310
High resolution limit [Å]	3.200	3.200
Rpim	0.090	0.030
Number of reflections	58821
<I/σ(I)>	7.3	2
Completeness [%]	97.2
Redundancy	6.5
CC(1/2)	0.986	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.2M ammonium sulfate, 0.1M Tris 7.5, 20% w/v PEG 5000 MME