6IE7

Crystal structure of ADCP1 tandem Agenet domain 1-2 in complex with H3K9me2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	41.472, 111.338, 68.144
Unit cell angles	90.00, 90.63, 90.00

Refinement procedure

Resolution	33.908 - 2.700
R-factor	0.1935
Rwork	0.190
R-free	0.25560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.258
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.700	7.320	2.700
Rmerge	0.066	0.040	0.295
Rmeas	0.081	0.049	0.363
Rpim	0.046	0.028	0.209
Total number of observations	38448
Number of reflections	15173	756	808
<I/σ(I)>	9
Completeness [%]	89.1	85.5	91.9
Redundancy	2.5	2.7	2.5
CC(1/2)		0.996	0.895

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	0.2M Ammonium acetate, 28% PEG 4000, 0.1M Sodium acetate pH 5.0