6IDR

Crystal structure of Vibrio cholerae MATE transporter VcmN in the bent form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-15
Detector	RAYONIX MX225HE
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	52.254, 93.742, 100.243
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.121 - 2.502
R-factor	0.2365
Rwork	0.233
R-free	0.26450
RMSD bond length	0.003
RMSD bond angle	0.780
Data reduction software	DIALS
Data scaling software	Aimless (0.5.23)
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	93.740	93.740	2.600
High resolution limit [Å]	2.500	9.000	2.500
Rmerge	0.232	0.046	0.906
Rmeas	0.252	0.050	0.995
Rpim	0.096	0.020	0.406
Total number of observations	118705
Number of reflections	17737	452	1919
<I/σ(I)>	5.9
Completeness [%]	99.8	99.9	98.5
Redundancy	6.7	6	5.9
CC(1/2)	0.989	0.997	0.635

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	PEG 300, sodium citrate, ammonium fluoride