6IDM
Crystal structure of Peptidoglycan recognition protein (PGRP-S) with Tartaric acid at 3.20 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2018-05-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 88.309, 101.149, 163.566 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.990 - 3.200 |
| R-factor | 0.19478 |
| Rwork | 0.192 |
| R-free | 0.26540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rt4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.990 | 3.280 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.630 | |
| Rmeas | 0.350 | |
| Number of reflections | 12435 | 853 |
| <I/σ(I)> | 4.27 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.88 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 12% PEG 3350, 100mM Sodium potassium tartarate |
