6ICX
Crystal structure of H7 hemagglutinin mutant AH-AGPL (V186G) from the influenza virus A/Anhui/1/2013 (H7N9)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.91847 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 115.318, 115.318, 293.318 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.098 - 2.399 |
| R-factor | 0.2676 |
| Rwork | 0.266 |
| R-free | 0.30040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4kol |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.511 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.555 | 2.490 |
| High resolution limit [Å] | 2.399 | 2.400 |
| Rmerge | 0.072 | 0.633 |
| Number of reflections | 29523 | 2900 |
| <I/σ(I)> | 21.69 | |
| Completeness [%] | 99.1 | |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M HEPES pH 7.0, 12% w/v Polyethylene glycol 3350 |
