6ICR
LdCoroCC mutant- C482A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-08 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.97625 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.480, 49.720, 45.730 |
| Unit cell angles | 90.00, 110.59, 90.00 |
Refinement procedure
| Resolution | 33.644 - 2.040 |
| R-factor | 0.2654 |
| Rwork | 0.263 |
| R-free | 0.30530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cx2 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.665 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.644 | 2.382 |
| High resolution limit [Å] | 2.040 | 2.300 |
| Rmerge | 0.044 | 0.066 |
| Rmeas | 0.047 | 0.071 |
| Number of reflections | 15839 | 1103 |
| <I/σ(I)> | 16.16 | 12.44 |
| Completeness [%] | 99.6 | 99.82 |
| Redundancy | 7.6 | |
| CC(1/2) | 0.999 | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | Lithium sulfate 0.8M, ammonium sulfate 0.85M, sodium citrate buffer 0.1M |
