6IC1

urate oxidase under 90 bar of krypton

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-14
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.999
Spacegroup name	I 2 2 2
Unit cell lengths	79.470, 94.939, 104.098
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.150 - 1.100
R-factor	0.11546
Rwork	0.115
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1r51
RMSD bond length	0.021
RMSD bond angle	2.160
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	FFT
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.150	1.160
High resolution limit [Å]	1.100	1.100
Rmerge	0.056	0.460
Rmeas	0.063	0.516
Rpim	0.028	0.231
Number of reflections	147453	20666
<I/σ(I)>	10.1	2.4
Completeness [%]	93.1	90
Redundancy	4.7	4.8
CC(1/2)	0.999	0.879

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	8	291	20 MicroL URATE OXIDASE (at 20 mg/ML+0.2MG/ML 8-AZAXANTHINE, 50mM TRIS) MIXED WIH 20 microL (TRIS + PEG 8000 4-8%).