6IBE
The FERM domain of Human EPB41L3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.915, 78.687, 98.981 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.590 - 1.450 |
| R-factor | 0.15562 |
| Rwork | 0.154 |
| R-free | 0.19994 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2he7 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.695 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.690 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.066 | 1.536 |
| Rmeas | 0.079 | 1.896 |
| Rpim | 0.043 | 1.098 |
| Number of reflections | 54827 | 2741 |
| <I/σ(I)> | 13.5 | 1.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.1 | 5.4 |
| CC(1/2) | 0.944 | 0.401 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 0.15 M ammonium nitrate, 0.1 M MES, 5% (v/v) ethylene glycol, 5% (w/v) PEG 2000, 5% (w/v) PEG 3350, 5% (w/v) PEG 4000, 5% (w/v) PEG 5000 MME |
