6IB7
Structure of wild type SuhB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-26 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.718, 46.031, 72.915 |
| Unit cell angles | 90.00, 125.40, 90.00 |
Refinement procedure
| Resolution | 36.975 - 2.245 |
| R-factor | 0.2172 |
| Rwork | 0.215 |
| R-free | 0.26050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qfl |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.736 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.245 | 2.245 |
| Number of reflections | 11994 | 1919 |
| <I/σ(I)> | 10.7 | 1.1 |
| Completeness [%] | 97.2 | 96.8 |
| Redundancy | 3.3 | 3.4 |
| CC(1/2) | 0.990 | 0.580 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 291 | 0.1M Tris-HCl, pH 8.3, 12% PEG 8000, |
