6IAP
structure of human NKp46 in complex with antibody NKp46-1 and NKp46-4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.858, 73.827, 128.518 |
| Unit cell angles | 90.00, 90.57, 90.00 |
Refinement procedure
| Resolution | 48.470 - 2.900 |
| R-factor | 0.191 |
| Rwork | 0.187 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oll |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.240 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.470 | 3.050 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Number of reflections | 28415 | 28397 |
| <I/σ(I)> | 5.2 | |
| Completeness [%] | 99.3 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M acid carboxylique, 0.1M Tris/bicine pH8.5, 50%P500MME/P20k |
