6IAK
The crystal structure of the chicken CREB3 bZIP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-15 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 137.900, 167.060, 115.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.640 - 3.950 |
| R-factor | 0.263 |
| Rwork | 0.261 |
| R-free | 0.30000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.670 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.640 | 4.090 |
| High resolution limit [Å] | 3.950 | 3.950 |
| Rmerge | 0.300 | 1.800 |
| Number of reflections | 12046 | 887 |
| <I/σ(I)> | 3.3 | 1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.5 | 6.5 |
| CC(1/2) | 0.930 | 0.760 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 50% v/v 2-Methyl-2,4- pentanediol (MPD), 100mM Tri-sodium citrate pH 5.6, 10mM Magnesium chloride (MORPHEUS screen) |
