6IAE
T. brucei IFT22 GDP-bound crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97891 |
| Spacegroup name | P 61 |
| Unit cell lengths | 56.015, 56.015, 263.085 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.510 - 2.490 |
| R-factor | 0.2234 |
| Rwork | 0.220 |
| R-free | 0.24420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.956 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.570 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.111 | 2.800 |
| Number of reflections | 16300 | 1558 |
| <I/σ(I)> | 18.5 | 0.9 |
| Completeness [%] | 99.3 | 96.1 |
| Redundancy | 19.7 | 17.5 |
| CC(1/2) | 0.998 | 0.363 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 277 | 15% (w/v) PEG6000, 50 mM NaCacodylate pH 7.0 and 200 mM CaAcetate |
