6IAE

T. brucei IFT22 GDP-bound crystal structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97891
Spacegroup name	P 61
Unit cell lengths	56.015, 56.015, 263.085
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.510 - 2.490
R-factor	0.2234
Rwork	0.220
R-free	0.24420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.956
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.570
High resolution limit [Å]	2.490	2.490
Rmerge	0.111	2.800
Number of reflections	16300	1558
<I/σ(I)>	18.5	0.9
Completeness [%]	99.3	96.1
Redundancy	19.7	17.5
CC(1/2)	0.998	0.363

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	277	15% (w/v) PEG6000, 50 mM NaCacodylate pH 7.0 and 200 mM CaAcetate