6IAA
hRobo2 ectodomain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 290.767, 81.569, 158.656 |
| Unit cell angles | 90.00, 94.00, 90.00 |
Refinement procedure
| Resolution | 78.650 - 3.600 |
| R-factor | 0.22571 |
| Rwork | 0.222 |
| R-free | 0.28676 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.426 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.650 | 3.740 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmeas | 0.161 | |
| Number of reflections | 43176 | 4487 |
| <I/σ(I)> | 6.6 | 1.1 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 3.93 | 4 |
| CC(1/2) | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | protein concentrated to 13.5 mg/ml and precipitant mixture containing 10% PEG 8000, 0.1M HEPES pH 7 - 7.125, 9.5% Ethylene glycol and 2% MPD |
