6IA1
urate oxidase under 120 bar of argon
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.77 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 80.889, 95.503, 105.229 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.480 - 2.360 |
| R-factor | 0.201 |
| Rwork | 0.198 |
| R-free | 0.26396 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1r51 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.999 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | FFT |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.500 | 2.670 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.088 | 0.750 |
| Number of reflections | 47439 | 3555 |
| <I/σ(I)> | 8.2 | 1.44 |
| Completeness [%] | 97.3 | 98.9 |
| Redundancy | 4.8 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.5 | 291 | 20 microliter of protein (15 mg/ml) mixed with 20 microliter of solution: Buffers: Tris 0.05M and PHOSPHATE 0.05M + 4% PEG 4000. |
