6I9I
Rift valley fever virus Gn in complex with a neutralizing antibody fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.590, 61.950, 78.910 |
| Unit cell angles | 81.01, 77.52, 84.74 |
Refinement procedure
| Resolution | 23.600 - 1.980 |
| R-factor | 0.20478 |
| Rwork | 0.203 |
| R-free | 0.24343 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4j02 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.422 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.600 | 2.030 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.072 | 0.651 |
| Number of reflections | 59882 | |
| <I/σ(I)> | 5.8 | 1.1 |
| Completeness [%] | 96.2 | 95.3 |
| Redundancy | 1.8 | |
| CC(1/2) | 0.980 | 0.670 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 20% w/v PEG 500, 0.1 M bis-Tris pH 6.5 |
