6I98

Structure of the ferrioxamine B transporter FoxA from Pseudomonas aeruginosa, apo state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-05
Detector	DECTRIS PILATUS 300K
Wavelength(s)	1.03332
Spacegroup name	P 63 2 2
Unit cell lengths	174.554, 174.554, 180.245
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	151.170 - 2.800
R-factor	0.2156
Rwork	0.213
R-free	0.25990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.021
RMSD bond angle	2.340
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.530	2.910
High resolution limit [Å]	2.800	2.800
Rmerge	0.206
Rmeas	0.226
Rpim	0.092
Number of reflections	40394	4492
<I/σ(I)>	10	1.6
Completeness [%]	99.9	100
Redundancy	10.7
CC(1/2)	0.997	0.547

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	1.8 M Ammonium sulfate, 0.1M HEPES pH 7.4