6I89

Crystal structure of Antirestriction ArdC protein from R388 plasmid. Metal-free structure.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.99989
Spacegroup name	H 3
Unit cell lengths	136.798, 136.798, 51.701
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	68.400 - 2.000
R-factor	0.173
Rwork	0.172
R-free	0.19900
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.844
Data reduction software	MOSFLM (7.2.2)
Data scaling software	SCALA (3.3.21)
Phasing software	PHENIX (1.11.1_2575)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.400	2.072
High resolution limit [Å]	2.000	2.000
Rmerge	0.063	0.319
Rmeas	0.070	0.373
Number of reflections	24347	3505
<I/σ(I)>	14.4	3.8
Completeness [%]	99.8	99
Redundancy	5.1
CC(1/2)	0.998	0.889

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0,1 M HEPES pH 7,5; 10% w/v Polyethylene glycol 6,000 and 5% v/v (+/-)-2-Methyl-2,4-pentanediol. Cryoprotected with 20% 2-methyl-2 4-pentanediol.