6I7E
Plasmodium falciparum Myosin A, Pre-powerstroke
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-17 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.979384 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 197.460, 197.460, 178.144 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.170 - 3.492 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6i7d |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.250 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 171.005 | 3.735 |
| High resolution limit [Å] | 3.492 | 3.492 |
| Number of reflections | 15557 | |
| <I/σ(I)> | 12.1 | |
| Completeness [%] | 59.5 | |
| Redundancy | 22.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 0.6 M K-Phosphate dibasic, 0.7 M Na-Phosphate monobasic |
