6I7D
Plasmodium falciparum Myosin A, post-rigor and rigor-like states
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2016-03-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.906019 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.520, 258.730, 103.090 |
| Unit cell angles | 90.00, 92.08, 90.00 |
Refinement procedure
| Resolution | 49.190 - 2.820 |
| R-factor | 0.19 |
| Rwork | 0.187 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2os8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.220 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.190 | 2.921 |
| High resolution limit [Å] | 2.820 | 2.820 |
| Number of reflections | 78760 | |
| <I/σ(I)> | 12.54 | |
| Completeness [%] | 98.8 | |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 277 | 18 % PEG 3350; 100 mM glycine |
