6I6U
Circular permutant of ribosomal protein S6, adding 9aa to N terminal of P81-82, L75A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-31 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97999 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 72.910, 43.920, 70.630 |
| Unit cell angles | 90.00, 117.18, 90.00 |
Refinement procedure
| Resolution | 62.830 - 1.570 |
| R-factor | 0.2103 |
| Rwork | 0.208 |
| R-free | 0.25630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ris |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.121 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.6.2) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 62.830 | 62.830 | 1.590 |
| High resolution limit [Å] | 1.570 | 8.580 | 1.570 |
| Rmerge | 0.071 | 0.051 | 0.943 |
| Rmeas | 0.077 | 0.057 | 1.022 |
| Rpim | 0.030 | 0.025 | 0.390 |
| Total number of observations | 188024 | ||
| Number of reflections | 28101 | 158 | 1367 |
| <I/σ(I)> | 11.9 | ||
| Completeness [%] | 99.9 | 86.3 | 100 |
| Redundancy | 6.7 | 4.7 | 6.8 |
| CC(1/2) | 0.998 | 0.996 | 0.772 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Calcium chloride dehydrate 0.1 M Tris pH 8.0 20% w/v PEG 6000 |
