6I69

Circular permutant of ribosomal protein S6, adding 5aa to C terminal of P97-3, L10A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97626
Spacegroup name	P 41 21 2
Unit cell lengths	34.810, 34.810, 135.177
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.710 - 1.200
R-factor	0.1386
Rwork	0.137
R-free	0.16860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ris
RMSD bond length	0.012
RMSD bond angle	1.149
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.5.32)
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.810	34.810	1.220
High resolution limit [Å]	1.200	6.560	1.200
Rmerge	0.057	0.046	0.482
Rmeas	0.058	0.047	0.493
Rpim	0.012	0.011	0.099
Total number of observations	657721
Number of reflections	27159	193	1279
<I/σ(I)>	26.7
Completeness [%]	99.7	88.8	99.2
Redundancy	24.2	17.5	23.8
CC(1/2)	1.000	0.999	0.943

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.02 M Magnesium chloride hexahydrate 0.1 M HEPES pH 7.5 22% w/v Poly (acrylic acid sodium salt) 5100