6I69
Circular permutant of ribosomal protein S6, adding 5aa to C terminal of P97-3, L10A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97626 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 34.810, 34.810, 135.177 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.710 - 1.200 |
| R-factor | 0.1386 |
| Rwork | 0.137 |
| R-free | 0.16860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ris |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.149 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 34.810 | 34.810 | 1.220 |
| High resolution limit [Å] | 1.200 | 6.560 | 1.200 |
| Rmerge | 0.057 | 0.046 | 0.482 |
| Rmeas | 0.058 | 0.047 | 0.493 |
| Rpim | 0.012 | 0.011 | 0.099 |
| Total number of observations | 657721 | ||
| Number of reflections | 27159 | 193 | 1279 |
| <I/σ(I)> | 26.7 | ||
| Completeness [%] | 99.7 | 88.8 | 99.2 |
| Redundancy | 24.2 | 17.5 | 23.8 |
| CC(1/2) | 1.000 | 0.999 | 0.943 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.02 M Magnesium chloride hexahydrate 0.1 M HEPES pH 7.5 22% w/v Poly (acrylic acid sodium salt) 5100 |
