6I5Z
Papaver somniferum O-methyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 111.148, 111.148, 302.380 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 101.000 - 3.000 |
| R-factor | 0.20721 |
| Rwork | 0.203 |
| R-free | 0.28932 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6i5q |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.725 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 151.190 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.099 | 1.873 |
| Rpim | 0.034 | 0.652 |
| Number of reflections | 44411 | 4590 |
| <I/σ(I)> | 16.3 | 1.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10 | 10.1 |
| CC(1/2) | 0.999 | 0.530 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | PEG 2000, sodium succinate, HEPES |
